GC-MS analysis, antidiabetic and antioxidant activity of methanolic extract of pluteus cervinus: an in vitro and in silico approach

Abstract

Pluteus cervinus is a mushroom species that demands a systematic study of its medicinal values. This study aims to explore the mycochemical contents of Pluteus cervinus extract, and evaluate their anti-diabetic and antioxidant potency. Twelve organic compounds were identified using Gas Chromatogram-Mass Spectroscopy(GC-MS) analysis of the methanolic extract. All the mycochemicals identified were evaluated for their drug-likeness, pharmacokinetics and bioactivity using admetSAR and molinspiration webservers. The extract was evaluated for the inhibitory effect of α-amylase and DPPH free radical scavenging ability. To further support the anti-diabetic and antioxidant characteristic, in silico molecular docking analysis was done for all the identified mycochemicals. It was found that, 2-(3,4-dimethoxyphenyl)-N-[4-(4-methoxyphenyl)-tetrahydropyran-4-ylmethyl]-acetamide, one of the compounds in the extract shares structural similarity and comparable docking binding energy with natural α-amylase inhibitor. Further, the 2-(3,4-dimethoxyphenyl)-N-[4-(4-methoxyphenyl)-tetrahydropyran-4-ylmethyl]-acetamide also showed high binding energy with Human peroxiredoxin 5 and has a structural relationship with natural antioxidants containing tetrahydropyran derivatives.

Graphical Abstract

Keywords:

• Pluteus cervinus
• Mycochemicals
• antidiabetic
• antioxidant
• in silico analysis
• molecular docking

Acknowledgements

The Authors thank Dr. M Kumar Assistant Professor, Department of Botony, Madras Christian College for helping in identifying the mushroom species. The authors thank SRM institute of science and Technology for the GC-MS analysis.

Disclosure statement

The authors declare no competing interests.