Dereplication approach for the first time isolation of tatarinowin a and pentadecanoic acid from Acorus calamus L. by using GC-MS

Abstract

In the present study, we report herein the isolation of cadinane-type sesquiterpenoid, tatarinowin A (ACH-6), and pentadecanoic acid (ACH-8) from petroleum ether extract of rhizome of Acorus calamus L. (Acoraceae) along with 6 other known compounds in this species. It is pertinent to mention here that this is the first report to stain these compounds in which dereplication approach based on GC-MS was applied to target unknown compounds ACH-6 and ACH-8 in A. calamus L. Derelpication approaches based on GC-MS is very useful technique in the area of drug discovery and have eminence potential to identify known and unknown compounds present in extracts of medicinal important plants. This technique can be used to expedite the process of purification of unknown compounds from different matrixes. The isolated compounds were identified with the help of inbuilt library search which reveals the presence of 17 known and 4 unknown compounds. Further, the structure elucidation of all isolated compounds was done using spectroscopy techniques. Also, the structure of ACH-6 was further confirmed by using the single-crystal X-ray diffraction technique.

Graphical Abstract

Keywords:

• Acorus calamus
• tatarinowin A
• pentadecanoic acid
• dereplication
• single-crystal X-ray diffraction technique

Acknowledgements

CK acknowledge AcSIR for their enrollment in Ph.D program. Authors are thankful to Director, CSIR-IIIM, Jammu for providing necessary facilities and support to carry out the work. Authors would like to thanks Dr. Bikarma Singh for identification of the plant material.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

We would like to acknowledge CSIR (New Delhi) for providing financial support for the work.