Component analysis of Xylaria sp. L1 sporocarps after solid-state fermentation by okara and its safety evaluation in mice and rats

Abstract

To explore the chemical components and nutrient components of Xylaria sp. L1 sporocarps, component analysis was characterized using UHPLC-LTQ-Orbitrap-MS/MS. Then, the acute toxicity and the subacute toxicity were conducted, respectively. A total of 38 compounds were detected and quantified. Meanwhile, Xylaria sp. L1 sporocarps had higher quantities of macronutrients, primarily Zn, which was remarkably higher than the human recommended daily values (p < 0.001). Importantly, no evidence of toxicity was observed in the mice after acute exposure to Xylaria sp. L1 sporocarps. In subacute toxicity studies, there were no significant differences in the body and organ weights. In the blood chemistry analysis, no significant changes occurred. Pathologically, neither histopathological changes nor gross abnormalities were observed. Thus, our study highlights the potential for using Xylaria sp. L1 sporocarps as novel food with Zn supplementation for humans.

Graphical Abstract

Keywords:

• Chemical component
• safety evaluation
• toxicology
• Zn supplementation

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the National Science Foundation Project of Anhui (1908085MC63), the National Key R&D Program of China (No. JZ2018ZDYF0541), the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (No. 2019L0037) and Shanxi Province Science Foundation for Youths (No. 201801D221285).