A new C₁₉-diterpenoid alkaloid in Aconitum georgei Comber

Abstract

Nine diterpenoid alkaloids were isolated from Aconitum georgei Comber belonging to the genus Aconitum in Ranunculaceae family. Their structures were determinated by using HR-ESI-MS and 1 D/2D NMR spectra as geordine (1), yunaconitine (2), chasmanine (3), crassicauline A (4), forestine (5), pseudaconine (6), 14-acetylalatisamine (7), austroconitine B (8), and talatisamine (9). Among them, compound 1 is a previously undescribed aconitine-type C₁₉-diterpenoid alkaloid, and compounds 3, and 5-9 have not previously been isolated from this species. The results of in vitro experiments indicated that new compound 1 possesses mild anti-inflammatory activity, which inhibited the production of NO in LPS-activated RAW 264.7 cells with an inhibition ratio of 29.75% at 50 μM.

Graphical abstract

Keywords:

• Aconitum georgei Comber
• Ranunculaceae
• diterpenoid alkaloid
• geordine
• anti-inflammatory

Acknowledgments

The authors would loke to thank Advanced Analysis and Measurement Center of Yunnan University for the sample testing service.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

Project supported by the National Natural Science Foundation of China (No. 31860095); the Natural Science Foundation of the Yunnan Province (202001BB050042); the Innovation and Entrepreneurship Training Program for college students of Yunnan University (202105113).