Development of inclusion complexes of 2-hydroxypropyl-β-cyclodextrin with Psidium guajava L. essential oil by freeze-drying and kneading methods for application as Aedes aegypti L. larvicide

Abstract

Inclusion complexes (ICs) of 2-hydroxypropyl-β-cyclodextrin with the essential oil (EO) from Seculo XXI cultivar of Psidium guajava were prepared using kneading (KN) and freeze-drying (FD) methods. The resulting ICs clusters have a nanometric size, with a diameter of approximately 80 and 40 nm for KN and FD, respectively. Complexation efficiency was 80.3% and 50.8% for KN and FD methods, respectively. The larvicidal activity of the EO in DMSO on A. aegypti had LC₅₀ and LC₉₀ values of 51.49 and 64.51 µg mL⁻¹, respectively. For the KN method, the toxicity corresponded to 77.54 and 107.29 µg mL⁻¹ for LC₅₀ and LC₉₀, respectively. FD method demonstrated toxicity at concentrations above 600 µg mL⁻¹. Thus, ICs enable the use of EO in breeding sites for A. aegypti, thus being potential products to be commercially exploited.

Graphical Abstract

Keywords:

• Inclusion complexes
• nanomaterial
• natural products
• guava tree
• dengue

Acknowledgments

The authors would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAP EMIG), Fundação de Amparo à Pesquisa e Inovação do Espírito Santo (FAP ES) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAP ES) for the financial support and the Microscopy Center of Universidade Federal de Minas Gerais and the and the Department of Physics of Universidade Federal de Viçosa for the characterization analysis.

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

We would like to thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Fundação de Amparo à Pesquisa e Inovação do Espírito Santo for financial support.