Chrysin inhibits the cell viability, induces apoptosis and modulates expression of genes related to epigenetic events in bladder cancer cells

Abstract

This study was conducted with the aim of exploring the molecular and cellular mechanisms of action of the chrysin (natural flavonoid compound) on bladder tumour cell lines with different status of TP53 (RT4, 5637 and T24). The cells were treated with different concentrations of chrysin (20, 40, 60, 80 and 100 µM) to analyze the cell viability, nuclear division index, mutagenicity, apoptosis rates and expression of genes related to epigenetic events (DNMT1, HAT1 and HDAC1). Results showed that the treatment with chrysin reduced the cell viability and caused apoptosis, regardless TP53. Moreover, in the TP53-mutated cell lines, chrysin modulated the expression of the DNMT1, HAT1 and HDAC1 epigenetic genes, which might be a plus to the death observed in the cells with p53 mutation.

Graphical Abstract

Keywords:

• Flavonoid
• bladder tumor
• cell death
• epigenetic events
• TP53
• IDN

Acknowledgements

This study was supported by Universidade Federal de Ouro Preto [grant number 23109.004080/2019–88], Coordenação de Aperfeiçoamento de Pessoal de Nível Superior [finance code 001], and Conselho Nacional de Desenvolvimento Científico e Tecnológico [grant number CNPq-310905/2020-6, CNPq Research Productivity Scholarship].

Author contribution

APBL carried out the experiments, contributed to the experimental design and data interpretation and wrote the manuscript. ASM supported the experiments. GMF supported the experiments. GNS was the advisor, contributed to the experimental design, as well as critically read the manuscript

Compliance with ethical standards

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by Universidade Federal de Ouro Preto [grant number 23109.004080/2019–88], Coordenação de Aperfeiçoamento de Pessoal de Nível Superior [finance code 001], and Conselho Nacional de Desenvolvimento Científico e Tecnológico [grant number CNPq-310905/2020-6, CNPq Research Productivity Scholarship].