Cytoprotective effect of Fridericia chica (Bonpl.) L.G. Lohmann extract associated with geranylgeraniol enriched-fraction from Bixa orellana L. on epithelial cells treated with bisphosphonate

Abstract

Bisphosphonates are drugs used to treat bone disorders. The chronic use of bisphosphonates is associated with the occurrence of medication-related osteonecrosis of the jaw (MRONJ). Previous data reported the positive effects of Geranylgeraniol on different cell types treated with Bisphosphonates. Foregoing work done by our research group demonstrated the wound healing capacity of Fridericia chica (Bonpl.) L.G.Lohmann standardized ethanol extract. Herein in vitro cytoprotective synergistic effect of the association of F. chica extract associated with an enriched geranylgeraniol fraction on keratinocytes exposed to zoledronic acid is reported. An association of F. chica at 1 and 5 µg/mL with geranylgeraniol at 15 µg/mL, increased cell viability by 73.5% and 71.1%, respectively. This treatment did not increase tumor cells viability; whereas the clonogenic potential assessment showed that, the association with F. chica (5 µg/mL) reversed the effects of zoledronic acid on the cells. This study provides data for a potential treatment for MRONJ.

Graphical Abstract

Keywords:

• Bisphosphonates
• Fridericia chica (Bonpl.) L.G.Lohmann
• Arrabidaea chica Verlot
• cell viability

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

CAPES, FAPESP grant number # 2017/21801-2 and CNPq, Brazil, grant numbers # 132448/2016-5, # 132207/2017-6, # 429463/2018-9. The authors A.L.T.G.R., J.E.C., S.O.S., and M.A.F thank CNPq for research productivity fellowship.