Two new triterpenoid glycosides from Bacopa monnieri and their cytotoxic activity

Abstract

Using combined chromatographic methods, two new triterpenoid glycosides, bacopasaponin K (1) and bacopasaponin L (2), along with eight known compounds, bacopaside IV (3), bacopaside VII (4), bacopasaponin E (5), bacoside A₃ (6), bacopasaponin F (7), bacopasaponin C (8), bacopaside I (9), and bacopaside II (10) were isolated from the methanol extract of the Bacopa monnieri. Their structures were elucidated by 1D-, 2D-NMR spectroscopic analysis, HR-ESI-MS and comparing with the NMR data reported in the literature. All these compounds were evaluated for their cytotoxic activity using the cell counting kit-8 (CCK-8) assay. Compounds 4, 6, 8, and 10 exhibited potential cytotoxic effects against human lung cancer cells (PC9) and human colon cancer cells (SW620).

Graphical Abstract

Keywords:

• Bacopa monnieri
• bacopasaponin
• triterpenoid glycoside
• bacopasaponin K
• bacopasaponin L

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Vietnam Academy of Science and Technology (Grant no. NVCC38.06/22-23); the Ministry of Education, Science and Technology, Republic of Korea (Grant no. NRF-2021K2A9A1A06086013 and 2022R1F1A1063364).