Seedpod extracts of Wrightia tinctoria shows significant anti-inflammatory effects in HepG2 and RAW-264.7cell lines

Abstract

W. tinctoria, an Indian herb Indrajao, has significant therapeutic potential. While studies have highlighted the anti-inflammatory potential of the leaves and bark of this plant, similar efficacy of the seed-pods remains unexplored. We demonstrate significant anti-inflammatory effects of the hexane fraction (Fr-B) of ethyl acetate extract of the seedpods in reducing lipopolysaccharide and palmitate mediated inflammation in RAW264.7 macrophages and HepG2 cells. GC-MS and NMR profiling of Fr-B revealed the existence of hexadecanoic acid, ethyl hexadecanoate, 9,12-octadecanoic acid, 9,12,15-octadecatrienoic acid, 9,12,15-octadecatrienoic acid ethyl ester, ethyl linoleate and octadecanoic acid ethyl esters.

Graphical Abstract

Keywords:

• Wrightia tinctoria
• lipopolysaccharide
• palmitic acid
• inflammation
• fatty acids
• HepG2
• RAW-264.7

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

Instrumentation support from CIF division of the institute is appreciated. This work was supported by grants from in-house projects (CSIR-IICB).