Cytotoxic effect of Cotinus coggygria extract on Hep3B cancer cell line

Abstract

Cotinus coggygria Scop. is a medicinal plant which contains a variety of precious phytochemical compounds applicable in traditional medicine. The aim of this study is to investigate the cytotoxic effect of Cotinus coggygria extract on Hep3B cancer cells. The cytotoxic effect was measured by MTT assay. Based on IC₅₀ values, the C. coggygria leaf extract showed cytotoxic effect on Hep3B cancer cells, which was found better at 24 h (IC₅₀ = 3.642) then at 48 h (IC₅₀ = 3.956). Also, the dose of C. coggygria extract showed negative correlation (p < 0.05) with cell viability of 24 h and 48 h and positive correlation (p < 0.05) with cytotoxic effect of 24 h and 48 h. The cytotoxic effects were concentration-time dependent. According to our study, C. coggygria extract had protective effect at 24 h on AML12 cell line and cytotoxic effect on the Hep3B cancer cell line, where it indicates that it may have an antitumorigenic effect.

Graphical Abstract

Keywords:

• Cotinus coggygria
• cytotoxic effect
• Hep3B
• AML12

Acknowledgements

The authors wish to thank Technology Research Development Application and Research Center (TUTAGEM) for the MTT assay. The authors are grateful to Dr. Necmettin Guler for the taxonomic identity of the plant. This work was supported financially by the Scientific Research Fund of Trakya University, Project number TUBAP-2019/159.

Disclosure statement

The authors declare that they have no conflict of interest.