Hemiactinomycin, an undescribed intermediate of actinomycin biosynthesis from an actinomycetes strain Streptomyces antibioticus H41-55

Abstract

Hemiactinomycin (1), an intermediate derivative of actinomycin biosynthesis, together with three known actinomycins (2-4) , were isolated from the ethanolic extract of Streptomyces antibioticus H41-55 fermentation mycelium by using various column chromatography. The structure of the derivative was established by extensive spectroscopic analysis, including HRESIMS, 1D, and 2D NMR spectroscopy. In addition, the anti-inflammatory activities of all the isolates were tested. The derivative (1) showed inhibiting NO release activities in LPS-induced RAW 264.7 macrophages with the IC₅₀ values of 15.41 ± 0.66 μM.

Graphical Abstract

Keywords:

• Actinomycetes
• hemiactinomycin
• anti-inflammatory
• biosynthesis

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This research was financially supported from the grant (2020A1515010389) of Natural Science Foundation Committee of Guangdong Province, and Special fund for scientific innovation strategy-construction of high-level GuangDong Academy of Agriculture Science (R2017YJ-YB2002).