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Natural Product Research
Formerly Natural Product Letters
Volume 38, 2024 - Issue 9
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Short Communications

A high performance thin layer chromatography (HPTLC) method for the quality assessment of agarwood (Aquilaria malaccensis) oil from Northeast India

, , , ORCID Icon & ORCID Icon
Pages 1634-1638 | Received 20 Oct 2022, Accepted 16 Dec 2022, Published online: 29 Dec 2022
 

Abstract

The high-value agarwood oil, largely used in perfumery is generally graded by the traditional method of sensorial assessment. The compositional complexity and variation made its quality control challenging. Besides, non-volatile contaminants and adulterants are the bottlenecks in gas-chromatographic detection. Herein, a HPTLC based technique was developed for the quality assessment of agarwood oil from Northeast India. A ‘marker band’ (anisylacetone and oxygenated sesquiterpene rich) on HPTLC profile, containing major peaks of the oil and characteristic agarwood aroma was quantified to assess the quality. The developed method was validated in terms of specificity, linearity, sensitivity, recovery and precision. The application of the method in test samples of three different grades indicated a positive correlation between ‘marker band’ quantity and oil quality. Its abundance in the superior grade oil was >50% and <20% in poor grade. It can be an efficient analytical tool for the quality assessment and grading of agarwood oil.

Graphical Abstract

Disclosure statement

The authors declare no conflict of interest in relation to this article.

Additional information

Funding

This work was supported by the CSIR-NEIST, Jorhat In-house Project (OLP 2073) and the Director, CSIR-NEIST, Jorhat.

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