Structure, anticoagulant and cytotoxic activity of a sulfated polysaccharide from green seaweed Chaetomorpha linum

Abstract

In this article, chemical structure and conformation in an aqueous solution of a new sulfated polysaccharide, P_CL, extracted from green seaweed Chaetomorpha linum were elucidated by SEC-MALL, IR, NMR and SAXS. The results indicated that the obtained polysaccharide is a sulfated arabinogalactan with a molecular weight of 223 kDa, and is mainly composed of →3,6)-α-D-Galp4S→ and →2)-α-L-Araf→ connecting together through 1→3 glycoside linkages. It has a broken rod-like conformation in solution with Rgc estimated as 0.43 nm from SAXS measurements. The polysaccharide exhibited a notable anticoagulant activity measured by the assays of activated partial thromboplastintime, thrombintime and prothrombine time as well as a significant cytotoxic activity against hepatocellular, human breast cancer, and cervical cancer cell lines.

Graphical Abstract

Keywords:

• Arabinogalactan
• Chaetomorpha linum
• small angle X-ray scattering
• anticoagulant activity
• cytotoxic activity

Acknowledgments

The synchrotron radiation experiments were performed under the approval of Japan Synchrotron Radiation Research Institute (Proposal No. 2014B1937). The authors would like to thank Professor Shinichi Kitamura at Osaka Metropolitan University for SEC-MALLS measurement.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This research is funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 104.01-2018.11.