Antitumor effects of Cypaliuruside F from Cyclocarya paliurus on HepG2 cells

Abstract

An undescribed dammarane triterpenoid saponin Cypaliuruside F was isolated from the leaves of Cyclocarya paliurus in our preliminary study. The MTT assay, flow cytometry, cell scratch, and DAPI staining were used to detect the antitumor effects of Cypaliuruside F on HepG2 cells. Subsequently, network pharmacology and molecular docking analysis were used to analyse the key targets of Cypaliuruside F against HCC. In addition, a Western blot was performed to determine the effects of Cypaliuruside F on the expression of key proteins in HepG2 cells. The experimental results indicated that the damarane triterpenoid saponin Cypaliuruside F from Cyclocarya paliurus inhibits the proliferation of HepG2 cells by inducing apoptosis and cell cycle arrest. These changes may promote the apoptosis of HepG2 cells by inhibiting the expression of mTOR, STAT3, and Bcl-2 while activating Bax.

Graphical Abstract

Keywords:

• Cyclocarya paliurus
• antitumor
• HepG2 cells
• network pharmacology

Disclosure statement

No potential conflict interest was reported by the authors.

Additional information

Funding

This work was supported by the [Natural Science Foundation of Guangxi Province] under Grant [No. 2018GXNSFAA281102].