RP–UHPLC–ESI–QTOF–MS analyses and evaluation of the antioxidant, antimicrobial, anti-diabetic and anti-Alzheimer activities of Linum tenue Desf

Abstract

The polyphenolic compounds of the n-butanol fraction of Linum tenue Desf. (BFLTe) were characterised by RP–UHPLC–ESI–QTOF–MS analyses with the main presence of 6,8-di-C-glucosyl naringenin (11.7%), vicenin 2-isomer 2 (8.18%), luteolin-7,3’-di-O-β-D-glucoside (7.18%), isovitexin (5.98%), luteolin-7-O-β-D-glucoside (5.713%), myricitrin (4.41%), luteolin-4’-O-β-D-glucoside (4.04%), chlorogenic acid (28.68%), 3-(2,6-dihydroxyphenyl)-4-hydroxy-6-methyl-3H-2-benzofuran-1-one (8.17%) and p-coumaric acid (4.0%.). The antioxidant capacity was evaluated using three complementary methods (DPPH, ABTS and Reducing power). Additionally, the antimicrobial activity was tested against eight bacterial strains and the fungi Candida albicans whereas the antidiabetic activity was performed against α-amylase. The anti-Alzheimer activity was tested by inhibiting the butyrylcholinesterase (BChE). The BFLTe showed, for the first-time, a good antioxidant potential in DPPH (IC₅₀:68.83 ± 2.74 µg/mL), ABTS (IC₅₀:48.73 ± 1.07 µg/mL) and Reducing power assays (A_0.50:99.98 ± 1.18 µg/mL) and a moderate antimicrobial activity with 250 and 500 µg/mL MICs values. Moreover, the fraction exhibited an excellent inhibition of the BChE (IC₅₀:33.00 ± 0.85 µg/mL) and α-amylase (IC₅₀:1093.13 ± 12.93 µg/mL).

Graphical Abstract

Keywords:

• Linum tenue Desf
• RP–UHPLC–ESI–QTOF–MS
• antioxidant
• anti-cholinesterase
• α-amylase inhibition
• antimicrobial

Acknowledgments

The authors gratefully acknowledge DGRSDT-MESRS, Algeria and National Center of Biotechnology Research, Constantine, Algeria

Disclosure statement

No conflict of interest was reported by the author(s).

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.