Unrefined Peanut Oil as a Lipid Source in Diets for Juveniles of Two-banded Seabream Diplodus vulgaris

Abstract

A feeding study with Two-banded Seabream Diplodus vulgaris was conducted to determine the effects of replacement of fish oil (FO) by unrefined peanut oil (PO) on growth performance, feed utilization, body composition, fatty acid composition, and serum biochemical and hematological variables. Three isonitrogenous (35.8%) and isoenergetic (21.15 kJ/g) diets were formulated by replacing dietary FO with PO at levels of level 0% (PO₀), 50% (PO₅₀), or 100% (PO₁₀₀). Fish were fed twice a day until satiation for an experimental period of 8 weeks. The best growth performance was observed in fish fed with the PO₀ and PO₅₀ diets. A significant increase was observed in hematocrit and mean corpuscular volume levels of fish fed with PO₅₀ diet compared with the other groups. Hemoglobin, mean corpuscular hemoglobin, total protein, albumin, and globulin were not affected by dietary PO treatment and did not differ among experimental groups. The glucose level was highest in the PO₁₀₀ group. Triglyceride and cholesterol levels were lower in fish fed diets with PO inclusions than in those fed the control diet. The fatty acid composition of fish was significantly affected by the experimental diets. Glutamic oxaloacetic transaminase, glutamic pyruvictransaminase, lactate dehydrogenase, and alkaline phosphatase were not affected by dietary PO treatment. The n-3:n-6 ratio in fish fed the PO₀ diet was also higher than in fish fed the PO-supplemented diets. The results of the present study showed that FO could be substituted by PO up to 50% in Two-banded Seabream diets without any negative effect on growth performance or serum biochemical and hematological features.

Received July 20, 2015; accepted September 14, 2015

ACKNOWLEDGMENTS

The authors thank Nail Üçyol and Hakan Baki for their help.