POTENTIAL OF BRASSICA JUNCEA IN ORDER TO TREAT TEXTILE—EFFLUENT—CONTAMINATED SITES

Abstract

Three plant species (Brassica juncea, Sorghum vulgare, and Phaseolus mungo) of different agronomic consequence were evaluated for the decolorization of the dyes from textile effluent. B. juncea, S. vulgare, and P. mungo showed textile effluent decolorization up to 79, 57, and 53%, respectively. A significant decrease in shoot and root height, but no significant injury, was observed in the case of P. mungo and S. vulgare. B. juncea (Indian mustard), the most tolerant and more effective metals accumulator than other tested agricultural plant species, showed enhanced growth with respect to the height of the shoot and root, 129 and 178%, respectively, when grown using original textile effluent. Textile effluent induced intracellular nicotinamide adenine dinucleotide reduced (NADH)–dichlorophenol indophenol reductase significantly in the case of S. vulgare and B. juncea with 209 and 194%, respectively. The extracellular riboflavin reductase activity was induced by 223% in the case of P. mungo as compared to control plants. Significant induction of intracellular laccase (266%) was observed in the case of B. juncea, indicating their crucial role for a potential metabolism and further degradation of the textile effluent. The metabolites were identified as napthalenesufamide (m/z 372) and 2-amino-4, 6-dichlorotriazine (m/z 167), when B. juncea was used to degrade a model dye, Reactive red 2.

KEY WORDS:

• textile effluent
• Brassica juncea
• laccase
• biodegradation
• accumulation

Notes

*P < 0.001

^$ P < 0.001

^aμg of DCIP/riboflavin reduced min⁻¹ mg protein⁻¹

^bUnits min⁻¹ mg protein⁻¹

*P < 0.001 by two tail P–values comparison test

^aμg of DCIP/riboflavin reduced min⁻¹ mg protein⁻¹

^bUnits min⁻¹ mg protein⁻¹

*P < 0.01

**P < 0.001 by two tail P-values comparison test

^aμg of DCIP/riboflavin reduced min⁻¹ mg protein⁻¹

^bUnits min⁻¹ mg protein⁻¹