Response of Antioxidative Enzymes and Apoplastic Bypass Transport in Thlaspi Caerulescens and Raphanus Sativus to Cadmium Stress

Abstract

A hydroponics experiment using hyperaccumulator Thlaspi caerulescens (alpine pennycress) and non-specific accumulator Raphanus sativus (common radish) was conducted to investigate the short-term effect of increasing Cd concentrations (0, 25, 50, 75, 100 μM) on metal uptake, chlorophyll content, antioxidative enzymes, and apoplastic bypass flow. As expected, T. caerulescens generally showed better resistance to metal stress, which was reflected by higher Cd accumulation within plant tissues with no signs of chlorosis, or wilt. Glutathione reductase (GR) and superoxide dismutase (SOD) activities in fresh leaves were monitored as the plant metal-detoxifying response. In general, both plant species exhibited an increase trend of GR activity before declining at 100 μM likely due to excessive levels of phytotoxic Cd. SOD activity exhibited almost a similar variation pattern to GR and decreased also at 100 μM Cd. For both plant species, fluorescent PTS uptake (8-hydroxy-l,3,6-pyrenetrisulphonic acid) increased significantly with metal level in exposure solutions indicating that Cd has a comparable effect to drought or salinity in terms of the gain of relative importance in apoplastic bypass transport under such stress conditions.

KEY WORDS:

• Cadmium
• Thlaspi caerulescens
• Raphanus sativus
• antioxidative enzymes
• PTS

ACKNOWLEDGMENT

We greatly acknowledge Prof. Jian Feng Ma from the Research Institute for Bioresources, Okayama University, Japan, for kindly providing seeds of T. caerulescens.

Saoussen Benzarti and Helmi Hamdi have contributed equally to this work.

Notes

*Pearson product-moment correlation coefficients (r) are significant at p ≤ 0.05.

*Pearson product-moment correlation coefficients (r) are significant at p ≤ 0.05.