Toward Protein Engineering for Phytoremediation: Possibilities and Challenges

Abstract

The combination of rational protein engineering and directed evolution techniques allow for the redesign of enzymes with tailored properties for use in environmental remediation. This review summarizes current molecular methods for either altering or improving protein function and highlights examples of how these methods can address bioremediation problems. Although much of the protein engineering applied to environmental clean-up employs microbial systems, there is great potential for and significant challenges to translating these approaches to plant systems for phytoremediation purposes. Protein engineering technologies combined with genomic information and metabolic engineering strategies hold promise for the design of plants and microbes to remediate organic and inorganic pollutants.

KEY WORDS:

• protein engineering
• directed evolution
• remediation
• enzymes
• plants
• microbes

ACKNOWLEDGMENT

Funding for this work was from the International Center for Advanced Renewable Energy and Sustainability (I-CARES), Washington University in Saint Louis.