Exogenous Cytokinin Treatments of an NI Hyper-Accumulator, Alyssum Murale, Grown in a Serpentine Soil: Implications for Phytoextraction

Abstract

Application of exogenous plant growth regulators was examined as a viable technique to increase the efficiency of plant metal extraction from contaminated soils. The aim of this study was to investigate the alteration of Ni phytoextraction by Alyssum murale, a Ni hyperaccumulator, following the application of cytokinins. The following parameters were investigated: Ni accumulation, plant growth, gas exchange, stomata behavior and the concentration of nonprotein thiols (glutathione, y-Glu-Cys, and phytochelatins). In a pot experiment, A. murale plants grown in a serpentine soil were treated with a mix of naturally occurring cytokinins. Results showed that Ni accumulation in plants ranged from 4000 to 7000 mg kg⁻¹ confirming the hyper-accumulation ability from the soil used. Cytokinin treatments produced a significant increase in plant biomass and transpiration rate whereas no significant variation in Ni accumulation or the concentration of non-protein thiols was observed. The results suggest that A. murale is a plant species sensitive to cytokinin treatment and that cytokinin treatment is potentially useful in increasing the phytoextraction capability by increasing biomass. Moreover, for first time, evidence was obtained that the Ni hyperaccumulation mechanism is independent of water flux and transpiration rate.

KEY WORDS:

• Alyssum murale
• plant growth regulators
• transpiration rate
• exogenous cytokinins
• metal hyperaccumulator
• serpentine soil
• phytochelatin

ACKNOWLEDGMENTS

The authors acknowledge Virginia Giansoldati for technical assistance. The research laboratory is supported by the Italian National Research Council.