Phytoremediation of Arsenic Contaminated Soil by Pteris Vittata L. II. Effect on Arsenic Uptake and Rice Yield

Abstract

A greenhouse experiment evaluated the effect of phytoextraction of arsenic from a contaminated soil by Chinese Brake Fern (Pteris vittata L.) and its subsequent effects on growth and uptake of arsenic by rice (Oryza sativa L.) crop. Pteris vittata was grown for one or two growing cycles of four months each with two phosphate sources, using single super phosphate (SSP) and di-ammonium phosphate (DAP). Rice was grown on phytoextracted soils followed by measurements of biomass yield (grain, straw, and root), arsenic concentration and, uptake by individual plant parts. The biomass yield (grain, straw and rice) of rice was highest in soil phytoextracted with Pteris vittata grown for two cycles and fertilized with diammonium phosphate (DAP). Total arsenic uptake in contaminated soil ranged from 8.2 to 16.9 mg pot⁻¹ in first growing cycle and 5.5 to 12.0 mg pot⁻¹ in second growing cycle of Pteris vittata. There was thus a mean reduction of 52% in arsenic content of rice grain after two growing cycle of Pteris vittata and 29% after the one growing cycle. The phytoextraction of arsenic contaminated soil by Pteris vittata was beneficial for growing rice resulted in decreased arsenic content in rice grain of <1 ppm. There was a mean improvement in rice grain yield 14% after two growing cycle and 8% after the one growing cycle of brake fern.

KEY WORDS::

• arsenic
• phytoextraction
• Pteris vittata
• rice
• grain
• straw
• content and uptake of arsenic

ACKNOWLEDGMENTS

The authors gratefully acknowledge Dr. K.V. Prabhu, Officer Incharge Phytotron Facilities, IARI, New Dehi. We acknowledge the help of Dr. M.C. Meena and Dr. B.S. Dwivedi during analysis of arsenic by AAS. The authors are indebted to the Indian Council of Agricultural Research for providing financial support for the study.