INFLUENCE OF SOIL PROPERTIES AND PHOSPHATE ADDITION ON ARSENIC UPTAKE FROM POLLUTED SOILS BY VELVETGRASS (HOLCUS LANATUS)

Abstract

Four kinds of soil material were used in a pot experiment with velvetgrass (Holcus lanatus). Two unpolluted soils: sand (S) and loam (L) were spiked with sodium arsenite (As III) and arsenate (As V), to obtain total arsenic (As) concentrations of 500 mg As kg⁻¹. Two other soils (ZS I, ZS III), containing 3320 and 5350 mg As kg⁻¹, were collected from Zloty Stok where gold and arsenic ores were mined and processed for several centuries. The effects of phosphate addition on plants growth and As uptake were investigated. Phosphate was applied to soils in the form of NH₄H₂PO₄ at the rate 0.2 g P/kg. Average concentrations of arsenic in the shoots of velvetgrass grown in spiked soils S and L without P amendment were in the range 18–210 mg As kg⁻¹ d.wt., whereas those in plants grown on ZS I and ZS II soils were considerably lower, and varied in the range 11–52 mg As kg⁻¹ d.wt. The addition of phosphate caused a significant increase in plant biomass and therefore the total amounts of As taken up by plants, however, the differences in As concentrations in the shoots of velvetgrass amended and non-amended with phosphate were not statistically significant.

KEY WORDS::

• As
• phytoremediation
• plant uptake
• soil
• solubility

ACKNOWLEDGMENTS

This study was supported by EU programs: “GRANT for PhD students—research supported by scholarships,” and Enterprising PhD Student—investment in the innovative regional development, as well as by Polish Ministry of Science and Higher Education (project N N305 320836).