EFFECT OF METAL TOLERANT PLANT GROWTH PROMOTING BACTERIA ON GROWTH AND METAL ACCUMULATION IN ZEA MAYS PLANTS GROWN IN FLY ASH AMENDED SOIL

Abstract

The present study was undertaken to examine the effect of the application of fly ash (FA) into Garden soil (GS), with and without inoculation of plant growth promoting bacteria (PGPB), on the growth and metal uptake by Zea mays plants. Three FA tolerant PGPB strains, Pseudomonas sp. PS5, PS14, and Bacillus sp. BC29 were isolated from FA contaminated soils and assessed for their plant growth promoting features on the Z. mays plants. All three strains were also examined for their ability to solubilize phosphate and to produce Indole Acetic Acid (IAA), siderophores, and hydrogencynide acid (HCN) production. Although inoculation of all strains significantly enhanced the growth of plants at both the concentration of FA but maximum growth was observed in plants inoculated with BC29 and PS14 at low level (25%) of FA concentration. The experimental results explored the plant growth promoting features of selected strains which not only enhanced growth and biomass of plants but also protected them from toxicity of FA.

KEY WORDS::

• fly ash
• pseudomonas
• bacillus

ACKNOWLEDGMENTS

The authors gratefully acknowledge The Director, Central Institute of Medicinal and Aromatic Research (CSIR), Lucknow for providing necessary laboratory facilities during research work. Department of Science and Technology (DST), New Delhi is also acknowledged for providing WOS-A fellowship to Dr. Kalpna V Kumar.