Screening of 19 Salix clones in effective phytofiltration potentials of manganese, zinc and copper in pilot-scale wetlands

Abstract

Willows (Salix spp.) have been regarded as one of interesting plants for phytofiltration of water contaminated with trace metals. In this paper, the clonal differences in tolerance and phytofiltration capacity of mixed metals (Mn, Zn, and Cu) were evaluated in greenhouse pilot-scale wetlands with a floating-support culture system. The results showed that broad clonal variations of biomass accumulation in response to mixed metals ranging from growth inhibition to stimulation. Clones differed in tolerance to multi-metals by tolerance index (TI) based on shoot and root biomass. We also found that wide variations in uptake and accumulation of three metals, which was related to species/clones and metal species. The willows showed high Mn and Zn translocation capacity from roots to leaves suitable for phytoextraction. In contrast, all clones had poor Cu translocation capacity, and Cu mainly retained in roots suitable for rhizofiltration. Among all Salix clones, clones SM30 and J903 had large phytofiltration potential for three metals with their high tolerance.

Keywords:

• Salix spp.
• manganese
• zinc
• copper
• phytofiltration

Acknowledgments

We thank Zhang Huaxian for excellent technical assistance.

Additional information

Funding

This work was funded by Natural Science Foundation of China (grant No. 31100513), the Key International Cooperative Project of CN-USA (Grant No. 2010DFB33960), key project from Zhejiang Science and Technology Bureau (Grant No. 2011C13015), the Key project of Ministry of Science and Technology of China (Grant No. 2012BAC17B02) and Fundamental Research Funds for the Central Universities.