Characterization of sorption sites and differential stress response of microalgae isolates against tannery effluents from ranipet industrial area—An application towards phycoremediation

ABSTRACT

Phycoremediation ability of microalgae namely Oscillatoria acuminate and Phormidium irrigum were validated against the heavy metals from tannery effluent of Ranipet industrial area. The microalgae species were cultured in media containing tannery effluent in two different volumes and the parameters like specific growth rate, protein content and antioxidant enzyme activities were estimated. FTIR spectroscopy was carried out to know the sorption sites interaction. The antioxidant enzymes namely superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) contents were increased in microalgae species indicating the free radical scavenging mechanism under heavy metal stress. SOD activity was 0.502 and 0.378 units/gram fresh weight, CAT activity was 1.36 and 0.256 units/gram fresh weight, GSH activity was 1.286 and 1.232 units/gram fresh weight respectively in the effluent treated microalgae species. Bio sorption efficiency for Oscillatoria acuminate and Phormidium irrigum was 90% and 80% respectively. FTIR analysis revealed the interaction of microalgae species with chemical groups present in the tannery effluent. From the results, the microalgae Oscillatoria acuminate possess high antioxidant activity and bio sorption efficiency when compared to Phormidium irrigum and hence considered useful in treating heavy metals contaminated effluents.

KEYWORDS:

• antioxidant enzymes
• heavy metals
• Oscillatoria acuminate
• Phormidium irrigum
• ranipet

Acknowledgments

The authors are thankful to the VIT University management for providing the infrastructure, constant support and encouragements. Authors also acknowledge Technology Business Incubator (TBI-VIT), sponsored by the Department of Science and Technology, for atomic absorption spectrometry analysis. The authors have declared no conflict of interest.