Morphophysiological, ultrastructural, and nutritional changes induced by Cu toxicity in young Erythrina fusca plants

ABSTRACT

Erythrina fusca is an important legume used for shade cover in cacao plantations in Brazil. Cacao plantations receive large quantities of copper (Cu)-containing agrochemicals, mainly for control of diseases. Therefore, Cu toxicity was investigated in seedlings grown in hydroponics with increasing concentrations of Cu (0.005–32 mg L⁻¹) in a greenhouse. Ultrastructural analyses showed cell plasmolysis in the root cortical area and changes in thylakoid membranes at 8 mg Cu L⁻¹ and higher. There were changes in epicuticular wax deposition on the leaf surface at the 16 and 32 mg Cu L⁻¹ treatments. Leaf gas exchanges were highly affected 24 hours after application of treatments beginning at 8 mg Cu L⁻¹ and higher Cu concentrations. Chemical analyses showed that Cu content in E. fusca roots increased as Cu concentration in the nutrient solution increased, whereas the shoot did not show significant changes. It is also observed that excess Cu interfered with Zn, Fe, Mn, Mg, K, P, and Ca content in the different E. fusca organs. Investigation of Cu toxicity symptoms focusing on morphophysiological, ultrastructural, gas exchange, and nutritional changes would be useful to alleviate Cu toxicity in E. fusca under field conditions, an important agroforestry species in cacao plantation.

KEYWORDS:

• heavy metal
• photosynthesis
• shade cover species

Acknowledgments

The authors thank the Electron Microscopy Center - Fiocruz, BA, Brazil, by MEV analyses and the technical assistance of Valéria Ferreira Fernandes of the Electron Microscopy Center-UESC.

Funding

V. L. Souza was supported by FAPESB (Fundação de Amparo a Pesquisa do Estado da Bahia).