Mobilization of cadmium from Festuca ovina roots and its simultaneous immobilization by soil in a root-soil-extractant system (in vitro test)

ABSTRACT

Mobilization of cadmium accumulated in Festuca ovina L. roots and simultaneous immobilization of this metal by soil were studied in three chambers connected into one system containing: (1) roots in an extractant solution, (2) soil in an extractant solution, and (3) extractant solution alone. Six extractants sterilized by filtration were used: 0.1 M NaNO₃ (NA), 0.1 mM desferrioxamine B (NA + DFOB) and 1 mM citric acid (NA + CA) in NA, and a water extract of soil (SE) supplemented with the same compounds. SE mobilized 53% of the Cd introduced to the system with roots. The addition of DFOB or CA to SE increased Cd extraction from roots by 17%, while the same compounds introduced to NA did not change mobilization of Cd (60% efficiency). Regardless of the extractant used, mobilization of Cd from roots was about 25% lower when extraction was done in a control system without soil. The metal released from roots was gradually immobilized by the soils loaded into all systems during a 4-day incubation. Sequential extractions of Cd from the soils showed that the metal released from roots with NA was stabilized mainly by soil Mn and Fe oxides, while that released with SE was stabilized by soil organic matter.

KEYWORDS:

• Cd desorption
• Cd resorption
• microbial activity

Funding

This work was financially supported by grant no. N N305 336334 from the Polish Ministry of Science and Higher Education.