Lead accumulation and distribution in maize seedlings: Relevance to biomass production and metal phytoextraction

ABSTRACT

Among trace metals, lead is a highly toxic contaminant, being hazardous to humans and animals. Application of maize plants for phytoremediation of polluted soils and waters has recently been of particular interest. The aim of this work is to investigate the Pb-phytoextraction potential of the maize cv. Tzariza used widely in Eastern European agriculture. Maize seedlings were exposed in a nutrient solution to 1–10000 µM of Pb²⁺ for 21 days. Lead accumulated mostly in conductive tissues and shoots at 0.1 mM and higher concentrations of Pb in growth medium. Pb at concentrations of 1 and 10 mM caused an increase in the superoxide anion level and the catalase activity in maize leaves. Lead ions were tolerable to maize seedlings within a concentration range up to 1000 µM of Pb^2+. The levels of lead in the nutrient solution above 1 mM resulted in inhibition of the growth of axial organs, decrease in leaf area, inhibition of water absorption, and reduction in accumulation of biomass. Theoretical considerations indicate that in the temperate climates of the phytoremediation with maize may allow annual removal up to 90 kg of Pb per km², depending on the initial level of soil contamination.

KEYWORDS:

• lead
• maize
• phytoremediation

Funding

This work was financially supported partially by the Statutory Funds of the Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University. The Jagiellonian University is a partner of the Leading National Research Center (KNOW) supported by the Ministry of Science and Higher Education.