Citric acid as soil amendment in cadmium removal by Salix viminalis L., alterations on biometric attributes and photosynthesis

Abstract

During the past decade, the target in cleaning polluted sites is an application of chelating agents, such as citric acid (CA), which is proposed as a good candidate in the promotion of phytoremediation. Among heavy metals, cadmium (Cd) is one of the most common and dangerous elements, which strongly disturbs morphophysiological properties in plants. A pot experiment was assessed to evaluate the influence of CA in Cd phytoremediation in alkaline soil by Salix viminalis (clone SV068). The effects of CA on Cd bioavailability, mobility, and distribution in plants, various morphometric measurements, and physiological performances as photosynthesis, transpiration, water use efficiency, and pigment content were tested. The highest Cd accumulation was evident after 60 days of growing, in plants subjected to combined treatment of CA with a higher dose of Cd. Application of CA showed a beneficial effect in maintaining the photosynthetic rate as well as gas exchange capacity in willows grown in Cd-contaminated soil. Furthermore, CA slightly increased plant growth and biomass production, depending on applied Cd dose and harvest period. A chelating agent like CA showed strong influence in plant response to combat Cd toxicity.

Keywords:

• Cd accumulation
• chelating agent
• photosynthetic performances
• phytoremediation
• willow

Acknowledgments

This research was conducted as a part of the project “Investigating the climate changes and their impact to environment: tracking impact, adaptation and reduction” III43007, financed by the Ministry of Education and Science of the Republic of Serbia.