Could acetone O-(4-chlorophenylsulfonyl)oxime be a copper chelating and antioxidative molecule on maize seedlings?

Abstract

The main purpose of study is to determine if Acetone O- (4-chlorophenylsulfonyl) oxime (AO) has a positive effect on maize seedlings under copper (Cu) stress or not. Seedlings were allocated to following experimental groups: 18-hour distilled water (DW) Control (C), ago 6-hour 0.66 mM AO + later 12-hour DW (AO), ago 6-hour DW + later 12-hour 1 mM Cu (Cu), ago 6-hour 0.66 mM AO + later 12-hour 1 mM Cu (AO + Cu). The results showed that AO + Cu caused approximately three times more copper accumulation compared to Cu treatment. AO and AO + Cu treatments significantly decreased membrane damage and H₂O₂ formation compared to its control. The proline content was significantly increased in AO and AO + Cu compared to its control. While the highest catalase, Guaiacol Peroxidase and superoxide dismutase activity was observed in Cu application, the highest ascorbate peroxidase activity was determined in AO application. It was observed that AO had a protective effect on chlorophyll content and RWC, but a positive effect on carotenoid content could not be determined. In addition, the effects of AO on the content of 17 phenolic substances in maize leaves were determined. In the light of the current findings, AO may prevent the formation of radical compounds.

Novelty statement

The fact that Acetone O-(4 chlorophenylsulfonyl) oxime (AO) caused approximately three times more copper accumulation indicates that it has a significant potential for phytoremediation studies. Despite the fact that AO resulted in approximately three times more copper accumulation, it is surprising that the Maize plant was able to continue its almost normal metabolic activities. AO alleviated the adverse effects of stress by affecting the hormones and antioxidant system of the plant. If AO is used with hyper accumulator plants, it may have the potential to obtain effective solutions in heavily copper-contaminated soils.

Keywords:

• Acetone O-(4-chlorophenylsulfonyl)oxime (AO)
• antioxidant system
• copper stress
• maize seedlings
• phenolic substances

Acknowledgments

Thanks to PhD Adem KORKMAZ, who synthesized the AO compound, and Sakarya Maize Research Institute, Turkey, that supplied the maize seeds, cultivar ADA-523.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was funded by Committee of Scientific Research Projects, Muş Alparslan University [Grant No: MŞÜ-BAP-20-TBMY-4902-02].