Phenanthrene stress response and phytoremediation potential of free-floating fern Azolla filiculoides Lam.

Abstract

In this study, the potential of Azolla filiculoides, a freshwater fern species, on phenanthrene phytoremediation and biodegradation was investigated. Furthermore, the effect of phenanthrene on growth performance, photosynthetic activity and biosynthesis, and accumulation of secondary metabolites of A. filiculodes were evaluated. Plants were grown in a nitrogen-free Hoagland and exposed to different phenanthrene concentrations (0, 1, 5, and 10 mg/L). Exposure to 10 mg/L phenanthrene caused a significant reduction (42%) in Azolla filiculoides growth compared to control on day 14. The photosynthetic pigment content of A. filiculoides treated with 1 and 5 mg/L was almost the same as the control, while 10 mg/L phenanthrene was significantly reduced. In comparison to unplanted controls, the biodegradation percentages obtained from the planted growth medium were found to be 88, 69, and 60%, respectively, for the application of 1, 5, and 10 mg/L phenanthrene. Data on plant growth, photosynthetic pigments, secondary metabolite contents, and biodegradation percentages indicated the tolerance level and the effective phytoremediation potential of A. filiculoides for phenanthrene was <10 mg/L. The results indicated that A. filiculoides is highly effective in phytoremediation of low concentrations of phenanthrene pollution in a short time.

NOVELTY STATEMENT

We explored the phenanthrene phytoremediation potential of freshwater fern Azolla filiculoides for the first time. Exposure to high phenanthrene induced accumulation of secondary metabolites, while reducing plant growth and photosynthetic pigment content. A. filiculoides is highly effective in phytoremediation of phenanthrene pollution at low concentrations in a short time. Biodegradation (≥60%) promoted by A. filiculoides indicates that this plant is a promising candidate for phenanthrene phytoremediation in aquatic environments.

Keywords:

• Azolla
• biodegradation
• polycyclic aromatic hydrocarbons
• photosynthetic pigments
• secondary metabolites

Disclosure statement

The authors report there are no competing interests to declare.

Additional information

Funding

This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) under Grant [number 113Z934].