Nucleotides Part LXXX: Synthesis of 3′-O Fluorescence Labeled Thymidine Derivatives and Their 5′-O-Triphosphates

Abstract

A new labeling technique attaching a fluorescent pteridine derivative (3, 5) via a linker onto the 3′-OH group of 5′-O-dimethoxytritylthymidine (7) was developed to lead to the conjugates 8 and 11. After detritylation to give 9 and 12, the final conversion into the corresponding 5′-triphosphates (13, 14), which were isolated as sodium salts, was performed by known methods.

Keywords:

• Isoxanthopterin derivatives
• fluorescent marker
• thymidine conjugates
• nucleoside 5′-triphosphates

Acknowledgments

Part of the special issue in honor of Vladimir Efimov.