Fast DNA Interstrand Cross-Linking Reaction by 6-Vinylpurine

Abstract

Oligonucleotides that incorporate a reactive moiety to form an interstrand cross-link have been widely studied for their potential toward inhibiting gene expression or as basic tools for chemical biology studies. The 6-vinylpurine (2) newly designed in the current study serves well as a new purine-base moiety for increasing cross-link reactivity to target cytosine. Thus, oligonucleotides containing 6-vinylpurine exhibit a more selective and much smoother DNA cross-linking ability to cytosine than the oligonucleotide analogs derived from 2-amino-6-vinylpurine (1) previously explored.

Keywords:

• DNA interstrand cross-link
• purine analogs
• oligonucleotide

Acknowledgments

We are grateful to Ms. S. Izumihara and Ms. S. Sanoki for their assistance with the experiments. This research is supported by KAKENHI (23790146) Grant-in-Aid for Young Scientists (B).