Plants Salvage Deoxyribonucleosides in Mitochondria

Abstract

Deoxyribonucleoside kinases phosphorylate deoxyribonucleosides into the corresponding 5′-monophosphate deoxyribonucleosides to supply the cell with nucleic acid precursors. In mitochondrial fractions of the model plant Arabidopsis thaliana, we detected deoxyadenosine and thymidine kinase activities, while the cytosol fraction contained six-fold lower activity and chloroplasts contained no measurable activities. In addition, a mitochondrial fraction isolated from the potato Solanum tuberosum contained thymidine kinase and deoxyadenosine kinase activities. We conclude that an active salvage of deoxyribonucleosides in plants takes place in their mitochondria. In general, the observed localization of the plant dNK activities in the mitochondrion suggests that plants have a different organization of the deoxyribonucleoside salvage compared to mammals.

Keywords:

• Arabidopsis thaliana
• nucleic acid precursors
• subcellular localization
• deoxyribonucleosides
• phosphorylation