Probenecid directly impairs activation of the canine P2X7 receptor

ABSTRACT

The current study aimed to determine if probenecid could directly impair the canine P2X7 receptor, a ligand-gated cation channel activated by extracellular adenosine 5′-triphosphate (ATP). Patch clamp measurements demonstrated that probenecid impairs ATP-induced inward currents in HEK-293 cells expressing canine P2X7. Flow cytometric measurements of ethidium⁺ uptake into HEK-293 cells expressing canine P2X7 showed that probenecid impairs ATP-induced pore formation in a concentration-dependent manner, with a half maximal inhibitory concentration of 158 µM. Finally, ELISA measurements revealed that probenecid impairs ATP-induced interleukin-1β release in dog blood. In conclusion, this study reveals that probenecid can directly impair canine P2X7 activation.

KEYWORDS:

• Purinergic receptor
• extracellular nucleotide
• probenecid
• gout
• monocyte
• cytokine
• dog

Funding

The Canine Research Foundation, Australian Companion Animal Health Foundation and American Kennel Club Canine Health Foundation funded this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.