Advancement in specific strand scission of DNA and evaluation of in-vitro biological assessment by pharmacologically significant tetraaza macrocyclic metal complexes constrained by triazole

Abstract

A novel ligand, tetra-azamacrocyclic, L(C₂₄H₁₆N₁₂O₂S₄) and its complexes with the structures, [MLCl₂] and [CuL]Cl₂ (where M = Ni(II), Fe(II); L = S,S′-[benzene-1,3-diylbis(4H-1,2,4-triazole-5,3-diyl)]bis{[(5-benzene-1,3-diyl-4H-1,2,4-triazol-3yl)sulfanyl] ethanethioate} were described and depicted by the analytical and spectral techniques. These studies revealed an octahedral geometry for Ni(II) and Fe(II) structures, while the Cu(II) complex exhibited a square planar structure. All the integrated metal structures were screened against targeted species of pathogenic fungi and bacterial strains to evaluate in vitro antimicrobial activities. The binding capability of the complexes with CT-DNA was considered by supporting detailed examination followed by viscosity measurements and thermal denaturation studies. The mode of DNA cleavage using gel electrophoreses revealed that the complexes possess photonuclease characteristics against pUC19 DNA under UV–visible irradiation. The newly synthesized novel Cu(II)-L complex has been proven to be a potential DNA cleavage agent and a potent inhibitor against both the bacterial and fungi strains.

Introduction

Keywords:

• Tetra-azamacrocyclic ligand
• metal complexes
• antimicrobial activity
• DNA binding and cleavage