A Novel Universal Linker for Efficient Synthesis of Phosphorothioate Oligonucleotides

Abstract

A versatile and conformationally preorganized universal linker molecule is reported here for efficient synthesis of phosphorothioate oligonucleotides. With respect to nucleoside loaded support, comparable yield and quality based on ion-pair LC-MS are obtained for both deoxy and 2′-O-methoxyethyl modified phosphorothioate oligonucleotides. No 3′-phosphate or phosphorothioate monoester or any modification of universal molecule still attached to oligonucleotide was observed.

Keywords:

• Oligonucleotides
• universal linker
• solid support
• phosphorothioate
• antisense