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Nucleosides, Nucleotides & Nucleic Acids Volume 26, 2007 - Issue 8-9
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Original Articles

Photoinduced Crosslinking of Double-helical DNA by Psoralen Covalently Linked to a Triple Helix-forming Oligonucleotide under Near-physiological Conditions

Hong Li School of Chemistry, University of Southampton, Highfield, Southampton, United Kingdom
,
Victoria J. Broughton-Head School of Biological Sciences, University of Southampton, Southampton, United Kingdom
,
Keith R. Fox School of Biological Sciences, University of Southampton, Southampton, United Kingdom
&
Tom Brown School of Chemistry, University of Southampton, Highfield, Southampton, United Kingdom
Pages 1005-1009 | Published online: 10 Dec 2007
 
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Abstract

Stable triplexes have been generated under near-physiological conditions by the introduction of the C and T base analogues 3-methyl-2-aminopyridine-2 ′-deoxyriboside and 5-(3-aminoprop-2-ynyl)-′-deoxyuridine into psoralen-conjugated triplex-forming oligonucleotides. After irradiation with UV light at 365 nm, photo-induced cross-linking of the TFO to double-helical DNA was observed by UV-melting analysis and fluorescence measurements.

Acknowledgments

This work was supported by grants from the European Union (SNIPER) and the BBSRC. All oligonucleotide synthesis was carried out by ATDBio Ltd. (www.atdbio.com).

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