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Nucleosides, Nucleotides & Nucleic Acids Volume 26, 2007 - Issue 8-9
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Original Articles

Photochemically Induced RNA and DNA Abasic Sites

Pascal A. Küpfer Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland
&
Christian J. Leumann Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland
Pages 1177-1180 | Published online: 10 Dec 2007
 
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Abstract

Two phosphoramidite building blocks were synthesized that can easily be deprotected by UV light to reveal natural abasic sites in oligoribonucleotides as well as in oligodeoxyribonucleotides. Another building block which releases a 2 ′-O-methylated abasic site upon UV radiation is also described.

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