Synthesis and Characterization of Tetra-End Linked Oligonucleotides Capable of Forming Monomolecular G-Quadruplexes

Abstract

The chemical synthesis of two new G-rich Tetra-End-Linked-oligodeoxyribonucleotides (TEL-ODNs) as well as ¹H-NMR and CD spectra of the corresponding monomolecular quadruplexes (IVa and IVb) has been reported. The new TEL-ODNs, characterized by the presence of short branches in the linker moiety, could be very useful for the achievement of monomolecular quadruplexes with predetermined strand orientation.

Keywords:

• TEL-ODN
• G-quadruplex
• NMR

This work is supported by Italian MURST (PRIN 2005). The authors are grateful to Centro di Servizi Interdipartimentale di Analisi Strumentale (CSIAS), for supplying the NMR facilities.