Abstract
We describe the preparation of two batches of a polymer support for the incorporation of folic acid into oligonucleotides. The method permits the regioselective attachment of a target nucleic acid sequence through its 3′-end to either the α-or γ-carboxyl group of L-glutamic acid, respectively. The supports have been tested in solid-phase synthesis of oligonucleotide-folate conjugates for cell delivery studies.
The project was supported by a grant from the EU FP6 (No. LSHC-CT-2004-503036, project PROTHETS) and the President of Russia Grant for Young Scientists (MK-2744.2005.4).