Stepwise Functionalization of Ribonucleopeptides: Optimization of the Response of Fluorescent Ribonucleopeptide Sensors for ATP

Abstract

A stable complex of a peptide and RNA, ribonucleopeptide (RNP), provides a new framework to construct a macromolecular receptor for small molecules. The RNP receptor functionalized by a fluorophore-labeled Rev peptide exerts an optical signal associated with the ligand binding events. Replacing the Rev peptide of the ATP-binding RNP with a fluorophore-modified Rev peptide affords a fluorescent ATP sensor.

Keywords:

• RNA
• peptide
• ribonucleopeptide
• fluorescent bisensor
• in vitro selection

This work was supported in part by the Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan to T. M. (No. 17310125 and No. 17026019).