Pyrene-Functionalized 2 ′-Amino-α-L-LNA as Potential Diagnostic Probes

Abstract

Oligodeoxyribonucleotides (ONs) containing two incorporations of 2 ′-N-(pyren-1-yl)acetyl-2 ′-amino-α-L-LNA monomer Y are sensitive probes for detection of single nucleotide polymorphisms (SNP) in DNA. In addition, the ability of ONs containing pyrene-functionalized 2 ′-amino-α-L-LNA monomers ( W-Z ) to stabilize duplexes with an abasic site is demonstrated.

Keywords:

• Single nucleotide polymorphisms
• abasic sites
• pyrene
• 2 ′-amino-α-L-LNA

We greatly appreciate funding from The Danish National Research Foundation.

Notes

^a T _m-values measured as the maximum of the first derivative of the melting curve (A ₂₆₀ vs. temperature) recorded in medium salt buffer ([Na⁺] = 110 mM, [Cl⁻] = 100 mM, pH 7.0 (NaH₂PO₄/Na₂HPO₄)), using 1.0 μM concentrations of the two complementary strands. ΔT _m/mod is measured relative to the duplex between the unmodified DNA and the strand containing the abasic site. See Figure 1 for structure of monomers.