Toward a Catalytic Site in DNA: Polyaza Crown Ether as Non-Nucleosidic Building Blocks in DNA Conjugates

Abstract

A number of functionalized polyaza crown ether building blocks have been incorporated into DNA-conjugates as catalytic Cu ²⁺ binding sites. The effect of the DNA-conjugate catalyst on the stereochemical outcome of a Cu ²⁺-catalyzed Diels-Alder reaction will be presented.

Keywords:

• Polyaza crown ether
• catalysis
• DNA-conjugates

Funding of this work was provided by The Nucleic Acid Center (NAC).

Notes

^a 10 mM sodium phosphate, 100 mM NaCl, 0.1 mM EDTA, adjusted to pH 7.0, concentration of 1 μM for each DNA strand.