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Nucleosides, Nucleotides & Nucleic Acids Volume 26, 2007 - Issue 10-12
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Original Articles

A Flexible Method for the Fabrication of Gold Nanostructures Using Oligonucleotide Derivatives

Ramon Eritja School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin, Ireland
,
Anna Aviñó School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin, Ireland
,
Beatriz G. de la Torre School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin, Ireland
,
Donald Fitzmaurice Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain and Institute for Research in Biomedicina (IRB), Barcelona, Parc Científic de Barcelona, Barcelona, Spain
,
Andrea Ongaro Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain and Institute for Research in Biomedicina (IRB), Barcelona, Parc Científic de Barcelona, Barcelona, Spain
,
Sarmiza Elena Stanca Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain and Institute for Research in Biomedicina (IRB), Barcelona, Parc Científic de Barcelona, Barcelona, Spain
,
Andrea DiSalvo Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain and Institute for Research in Biomedicina (IRB), Barcelona, Parc Científic de Barcelona, Barcelona, Spain
,
Brendan Maninng Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain and Institute for Research in Biomedicina (IRB), Barcelona, Parc Científic de Barcelona, Barcelona, Spain
&
Daniela Iacopino Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain and Institute for Research in Biomedicina (IRB), Barcelona, Parc Científic de Barcelona, Barcelona, Spain
 show all
Pages 1605-1609 | Published online: 05 Dec 2007
 
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Abstract

Several linear and branched DNA structures from 80–200 nm with a biotine molecule in the middle have been prepared. These structures have been decorated by addition of positively charged gold nanoparticles carrying 4-(dimethylamino)pyridine ligands. Streptavidin binds to the central biotine molecule introducing a 20 nm gap in the structure in which a biotinylated nanoparticle can be introduced. The simplest structure (80 nm, linear) is formed by 4 oligonucleotides. By changing some of these components changes on length, shape, and recognition system easily can be introduced.

Project financed by E.E.C.C., IST-1999-11974, BIOAND and STRP 014006 Nano3D and Spanish Ministry of Education BFU2005-23719-E, NAN2004-09415-C05-03 and BFU2004-02048.

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