Structure and Stability of an Oligonucleotide Containing 2′-Deoxy-6-thioguanosine

Abstract

Structure and stability of the self-complementary octamers d(AAACGTTT) and d(AAACs⁶GTTT) have been evaluated by one and two-dimensional NMR and UV techniques. Temperature dependence studies by NMR and UV indicate the presence of 2′-deoxy-6-thioguanosine (ds⁶G) acts to destabilize duplex formation in the octamer by about 10 to 15°C. Comparison of octamers indicate the presence of ds⁶G octamer acts to induce structural perturbations that are not localized to the central dC-ds⁶G base pairs but affect the overall structure of the entire octamer.