Flow‐Injection Spectrophotometric Determination of Dipyrone in Pharmaceutical Formulations Using Ammonium Molybdate as Chromogenic Reagent

Abstract

A simple and rapid flow‐injection spectrophotometric method is reported for the determination of dipyrone in pharmaceutical formulations. The method is based on the reaction of dipyrone with ammonium molybdate in acidic medium to produce blue molybdenum, which was detected spectrophotometrically at 620 nm. The analyte was determined in a single‐line flow system. The calibration curve obtained was linear in the range of 5×10⁻⁴ to 8×10⁻³ mol L^–1 for dipyrone concentration and the precision (s _r=1.7%) was satisfactory. The method proved to be selective and adequately sensitive. Application of the method to the analysis of pharmaceutical samples resulted in excellent accuracy; the percent mean recoveries were in the range 95.3%–101% and relative errors less than 5.0% for five pharmaceutical formulations were found.

Keywords:

• Dipyrone
• flow injection analysis
• ammonium molybdate
• pharmaceutical formulation
• blue molybdenum

Acknowledgments

The authors gratefully acknowledge financial support from Brazilan foundations (CNPq, CAPES and FAPESP).