Effects of In Ovo Exposure to PCBs 126 and 77 on Mortality, Deformities and Post-hatch Immune Function in Chickens

Abstract

In laboratory experiments, planar PCBs produce immune organ atrophy in chicken embryos. To study the immunotoxic effects of PCBs in birds, the coplanar congeners 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) and 3,3′,4,4′-tetrachlorobiphenyl (PCB 77) were injected into the air cell of fertile white leghorn chicken eggs before incubation at doses of 0.25 and 0.5 ng/g egg PCB 126 and 0.64 ng/g egg PCB 77. Mortality and deformities were assessed during incubation of the eggs, and immune function was analyzed post-hatch using phytohemagglutinin (PHA) skin test for T-cell mediated immunity, antibody titers to sheep red blood cells (SRBC), mitogenesis of peripheral blood lymphocytes, and immune organ mass and cellularity. Exposure to 0.25 ng/g PCB 126 elevated mortality (61% and 69%) and deformities (31% and 32%), three or more times higher than controls. Two-fold suppression of antibody titers was observed in 28 day old chicks exposed to PCB 126 or PCB 77. No consistent alterations in PHA skin response or lymphocyte proliferation were observed. In 14 day old chicks in experiment two, PCB 126 decreased thymus and bursa cellularity by 33% and 35%, respectively. Immune organ atrophy was transient, recovering to control levels by 42 days of age. These experiments demonstrate that PCB 126 and 77 suppress antibody responses in juvenile chickens following an in ovo PCB exposure. Results reinforce the need for measuring multiple immune endpoints to detect immunotoxicity.

Acknowledgements

The authors appreciate the technical assistance of Elissa Reaves, Kendra Goff and Russell Garrett, and the comments of three anonymous reviewers. Funding was received from US EPA Grant GR825216-01-0.

Notes

Goff, K. F., 2002 Developmental effects of PCBs on apoptosis and the primary immune organs in white leghorn chicken embryos. Ph.D. Dissertation. Dayton, OH: Wright State University.

Pekarik C., Weseloh D. V., Barrett G. C., Simon M., Bishop C. A., Pettit K. E. 1998. An atlas of contaminants in the eggs of fish-eating colonial birds of the Great Lakes (1993–1997), volume II: Accounts by chemical. Technical report series no.321, Canadian Wildlife Service, Ontario Region.