Effects of Produced Water on Reproductive Parameters in Prespawning Atlantic Cod (Gadus morhua)

Abstract

Produced water (PW) discharged from offshore oil industry activities contains substances that are known to contribute to a range of mechanisms of toxicity. In the present study selected reproductive biomarkers were studied in prespawning Atlantic cod (Gadus morhua) exposed to PW. The fish were exposed for 12 wk within a continuous flow-through system at realistic environmental near-field concentrations. Concentrations of polyaromatic hydrocarbon (PAH) and alkylphenol (AP) compounds were analyzed by gas chromatography with mass spectrometric detection measurement, as were PAH and AP metabolites in fish bile for verification of exposure conditions and presence of compounds in PW. A suite of reproductive biomarkers (vitellogenin, zona radiata protein, and plasma steroid concentrations) and histological alterations of the gonads were determined. Results showed that exposure to sufficiently high levels of PW produced an increase in vitellogenin levels in female fish compared to the control. Impaired oocyte development and reduced estrogen levels were also observed in PW-exposed female fish. In male fish testicular development was altered, showing a rise in amount of spermatogonia and primary spermatocytes and a reduction in quantity of mature sperm in the PW-exposed fish compared to control. Data indicate that sufficiently high levels of PW have the potential to adversely affect the reproductive fitness of cod.

Acknowledgments

This study was funded by Total E&P Norway, ConocoPhillips Scandinavia, and the Norwegian Oil Industry Association (OLF). The authors are indebted to Bodil K. Larsen, Brit F. Godal, Nadia Aarab, Kjell Birger Øysæd, and Atle Nævdal (IRIS) for technical assistance. The National Veterinary Institute, Sandnes, is acknowledged for preparation of microscopy slides.