Estrogen Mimetic 4-tert-Octylphenol Enhances Ige-Mediated Degranulation of Rbl-2H3 Mast Cells

Abstract

Allergic diseases such as asthma have been on the rise in recent decades. Environmental or occupational exposure to estrogenic synthetic chemicals is suspected to be a contributing factor, and previous experimental studies indicated that estradiol and some xenoestrogens increase allergic signaling responses, such as degranulation, in immune cells. In the current study, data showed that the estrogen mimetic 4-tert-octylphenol (4tOP) enhances immunoglobulin (Ig) E-mediated degranulation of mammalian mast cell line RBL-2H3 (RBL). At the noncytotoxic concentrations 10–20 μM, 4tOP significantly increased degranulation in antigen (Ag)-activated RBLs but exerted no marked effect on spontaneous levels. Our data suggest that the industrial chemical 4tOP has the potential to enhance allergic disease in individuals who are exposed.

Acknowledgments

This research was funded by a Research Starter Grant in Pharmacology/Toxicology from the PhRMA Foundation, by the Maine Agricultural & Forest Experiment Station Grant Number ME08004-10, and by University of Maine startup funding. R.H.K. benefited from funding from Maine's Graduate School of Biomedical Sciences. We are grateful to Drs. David Holowka and Barbara Baird for the RBL cells and DNP-BSA. We thank Zsolt Kormendy for help with experiments and Andrew Abovian, Hina Hashmi, Sarah McGillicuddy, Zachary Tranchemontagne, Alejandro Velez, and Lisa Weatherly for literature searches. We thank Andrew Abovian and Benett Trinh for help with equipment and orders. This is Maine Agricultural & Forest Experiment Station publication number 3283.